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Glycogen Metabolism and Glycogen storage Diseases

Glycogen Metabolism and Glycogen storage Diseases

GLYCOGEN:
Glycogen is the major storage carbohydrate in animals, corresponding to starch in plants; it is a branched polymer of α-D-glucose. It is stored mainly in liver and muscle. Due to more muscle mass, the quantity of glycogen in muscle (250gm) is about 3 times higher than that in the liver (75 gm).
Functions:

Liver glycogen helps to maintain blood glucose level: Liver glycogen …

Ketone bodies, Ketosis and Ketoacidosis

Ketone bodies, Ketosis and Ketoacidosis

Ketone bodies, produced mainly in the mitochondria of liver cells from acetyl-CoA enter blood, provide much of the energy to heart tissue, and during starvation to the brain. They include 3 water soluble chemicals namely: acetone, acetoacetate and β-hydroxybutyrate (not a true ketone). The levels of acetone are much lower than those of the other 2 ketone bodies; it cannot …

Polymerase Chain Reaction: Principles and Uses of PCR

Polymerase Chain Reaction: Principles and Uses of PCR

The Polymerase Chain Reaction (PCR) is an invitro method of DNA amplification that can rapidly clone (amplify) DNA samples as small as a single molecule. If a length of DNA is mixed with the 4 nucleotides (A, T, C and G), and the enzyme DNA polymerase, then the DNA will be replicated many times. Developed in 1983 by Kary Mullis, …

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